| 產(chǎn)品編號(hào) | bsm-33033M |
| 英文名稱 | Mouse Anti-GAPDH antibody |
| 中文名稱 | 3-磷酸甘油醛脫氫酶（內(nèi)參）單克隆抗體 |
| 別 名 | 38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogenase liver;Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10. |
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Specific References (55) | bsm-33033M has been referenced in 55 publications.
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| 產(chǎn)品類型 | 內(nèi)參抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 新陳代謝 |
| 抗體來(lái)源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | 4F8 |
| 交叉反應(yīng) | Human,Mouse,Rat,Pig,Hamster (predicted: Rabbit,Sheep,Chicken,Dog,Monkey) |
| 產(chǎn)品應(yīng)用 | WB=1:10000-1:100000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinded Human GAPDH |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Loading Control Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subunit: Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with EIF1AD, USP25, PRKCI and WARS. Subcellular Location: Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions. Post-translational modifications: S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus. ISGylated (Probable). Sulfhydration at Cys-152 increases catalytic activity. Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. SWISS: P04406 Gene ID: 2597 Database links: Entrez Gene: 374193 Chicken Entrez Gene: 2597 Human Entrez Gene: 100042025 Mouse Entrez Gene: 14433 Mouse Entrez Gene: 317743 Zebrafish Omim: 138400 Human SwissProt: P00356 Chicken SwissProt: P04406 Human SwissProt: P16858 Mouse SwissProt: Q5XJ10 Zebrafish GAPDH蛋白幾乎在所有組織中都高水平表達(dá)，廣泛用作Western blot蛋白質(zhì)標(biāo)準(zhǔn)化的內(nèi)參,是很好的內(nèi)參抗體。 GAPDH 作為管家基因在同種細(xì)胞或者組織中的蛋白質(zhì)表達(dá)量一般是恒定的。在實(shí)驗(yàn)中，可能存在總蛋白濃度測(cè)定不準(zhǔn)確；或者蛋白質(zhì)樣品在電泳前上樣時(shí)產(chǎn)生的樣品間的操作誤差；這些誤差需要通過(guò)測(cè)定每個(gè)樣品中實(shí)際轉(zhuǎn)到膜上的GAPDH的含量來(lái)進(jìn)行校正，所以一般的western實(shí)驗(yàn)都需要進(jìn)行內(nèi)參設(shè)置。具體校正的方法就是將每個(gè)樣品測(cè)得的目的蛋白含量與本樣品的GAPDH含量相除，得到每個(gè)樣品目的蛋白的相對(duì)含量。然后才進(jìn)行樣品與樣品之間的比較。 甘油醛-3-磷酸脫氫酶（Glyceraldehyde 3 phosphate dehydrogenase，GAPDH）是糖酵解（glycolysis）過(guò)程中的關(guān)鍵酶。除了在胞質(zhì)中作為糖酵解的酶以外，有證據(jù)表明哺乳動(dòng)物細(xì)胞中的GAPDH參與了多種胞內(nèi)生化過(guò)程，包括膜融合（membrane fusion）、微管成束（microtubule bundling）、磷酸轉(zhuǎn)移酶（phosphotransferase）激活、核內(nèi)RNA出核、DNA復(fù)制與DNA修復(fù)。一些生理因素，諸如低氧（hypoxia）和尿糖（diabetes），可以增加GAPDH在特定細(xì)胞中的表達(dá)。GAPDH存在于幾乎所有的組織中，以高水平持續(xù)表達(dá)。 GAPDH（甘油醛-3-磷酸脫氫酶）是參與糖酵解的一種關(guān)鍵酶，由4個(gè)30－40kDa的亞基組成. |
| 產(chǎn)品圖片 |
Sample:
Lane1: Skin (Mouse) Lysate at 40 ug
Lane2: Testis (Mouse) Lysate at 40 ug
Lane3: Adrenal gland (Mouse) Lysate at 40 ug
Primary: Anti-GAPDH (bsm-33033M) at 1/1 000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Sample:
Lane 1: Rat Cerebrum tissue lysates
Lane 2: Rat Heart tissue lysates
Lane 3: Human A549 cell lysates
Lane 4: Human Jurkat cell lysates
Lane 5: Human Huvec cell lysates
Lane 6: Human Hela cell lysates
Lane 7: Human U2os cell lysates
Lane 8: Human HepG2 cell lysates
Primary: Anti- GAPDH (bsm-33033M) at 1/50000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Sample:
Lane 1: Human HEK293 cell lysates
Lane 2: Human Hela cell lysates
Lane 3: Human SH-SY5Y cell lysates
Lane 4: Mouse NIH/3T3 cell lysates
Lane 5: Hamster CHO cell lysates
Lane 6: Rat Brain tissue lysates
Primary: Anti-GAPDH (bsm-33033M) at 1/200000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (ascites of bsm-33033M-4E8) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (ascites of bsm-33033M-4E8) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human stomachc); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (bsm-33033M ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (bsm-33033M ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (bsm-33033M ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GAPDH-Loading Control) monoclonal Antibody, Unconjugated (bsm-33033M) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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